Transformation of Gossypium hirsutum and Gossypium barbadense by Rhizobium rhizogenes: Hairy root induction and gossypol production. Stephanie Moss , University of New Orleans. Gossypol and its methylated derivatives are produced in the leaves, seeds, stems, and roots of cotton plants. Although gossypol is toxic to many animals, other uses for gossypol are actively being investigated.
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Hairy root culture , also called transformed root culture , is a type of plant tissue culture that is used to study plant metabolic processes or to produce valuable secondary metabolites or recombinant proteins, often with plant genetic engineering. A naturally occurring soil bacterium Agrobacterium rhizogenes that contains root-inducing plasmids also called Ri plasmids can infect plant roots and cause them to produce a food source for the bacterium, opines , and to grow abnormally. The neoplastic roots produced by A. Currently the main constraint for commercial utilization of hairy root culture is the development and up-scaling of appropriate bioreactors vessels for the delicate and sensitive hairy roots. Hairy root cultures can be used for phytoremediation , and are particularly valuable for studies of the metabolic processes involved in phytoremediation. Further applications include detailed studies of fundamental molecular, genetic and biochemical aspects of genetic transformation and of hairy root induction. The Ri plasmids can be engineered to also contain T-DNA , used for genetic transformation biotransformation of the plant cells.
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Four graduate students are conducting their MS Biotechnology thesis work on Scutellaria biotechnology. These students have undergone required training in the area of cryopreservation, phytochemical screening, and hairy root culture research. Provided opportunities for PI and collaborators to attend meetings in the relevant field, learn and execute better experiments. This has helped in securing more grants for Scutellaria research. How have the results been disseminated to communities of interest?
Eunice Jessie Allan, J. Eeswara, A. Jarvis, Anne Jennifer Mordue, E. Morgan, Trevor Stuchbury.